Composite

Part:BBa_J100415

Designed by: Caroline Willis, Tatianna Travieso   Group: Campbell M Lab   (2018-06-21)


Modified T7 Promoter Gal4 Binding Site in pClone Red

Modified T7 promoter (J100404) in which "TAATAC" has been removed and the 17 base pair sequence of the Gal4 binding site has replaced it inserted into pClone Red (J119137). This encodes for RFP when inserted in into pClone. When present the Gal4 protein will bind to this site.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1448
    Illegal AgeI site found at 1560
  • 1000
    COMPATIBLE WITH RFC[1000]


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