Measurement

Part:BBa_J100400

Designed by: Malcolm Campbell   Group: Campbell_M_Lab   (2018-05-18)


pClone mScarlet + promoter "blue"

Started with pClone mScarlet (J100397) and performed golden gate assembly (GGA) on it using oligos for the promoter J100371 from the PM "blue" Bio113 spring 2018 group.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 917
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 917
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 917
    Illegal BamHI site found at 863
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 917
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 917
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None