Regulatory

Part:BBa_J100369:Design

Designed by: David Thole   Group: Campbell M Lab   (2018-02-22)


Promoter designed to efficiently transcript RFP gene in E. Coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Before choosing our sequence, we had to consider the effect of the restriction enzymes on the sticky ends that would be created. This was incorporated into our part as extra ends.


Source

This part was found in the paper "Indra Bervoets, Maarten Van Brempt, Katleen Van Nerom, Bob Van Hove, Jo Maertens, Marjan De Mey, Daniel Charlier; A sigma factor toolbox for orthogonal gene expression in Escherichia coli, Nucleic Acids Research, , gky010, https://doi.org/10.1093/nar/gky010" regarding orthogonal gene expression.

References