Part:BBa_J100339:Design
Mutated RBS
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Using Mfold, we analyzed the folding of various sequences. We searched for sequences with a more positive ΔG when comparing the binding of the RBS to the anti-RBS. The closer the ΔG to 0, the less exothermic the reaction will be, and therefore the less likely this binding will occur. The ΔG values from our previous sequence imply that the binding of our RBS to the anti-RBS would be much more likely than it binding to the 16S rRNA. The new RBS that we are proposing has a greater ΔG when binding to the anti-RBS than when binding to the 16S rRNA. Using this data, we are hypothesizing that our RBS will be stronger than the control from the paper, and greatly improve translation of the RFP gene.
Source
"rClone: A Synthetic Biology Tool That Enables the Research of Bacterial Translation" et. al. Eckdahl