Part:BBa_J100312:Experience

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Applications of BBa_J100312

We intend to use this part as a negative control for repClone Red (BBa_J100205) constructs with various manipulations of the TetR promoter. We compared red fluorescence of E. coli transformed with plasmids containing this part with 0 ng/mL and 400 ng/mL anhydrotetracycline. The fluorescence in relative fluorescence units, controlled for cell density, is shown below. We also tested part BBa_J100306, which contains the wildtype TetR promoter, under the same conditions. We see that the total fluorescence with the scrambled promoter is greater than that of the wt promoter, but that there was no increase in fluorescence by the addition of aTc as there was for cells containing wt.

Fluorescence by J100306 without aTc does not appear on the graph but was quantifiable. See data table below.

The numerical data are displayed, including the raw data for each treatment and ratio of RFU with aTc to RFU without aTc (a value of 1 would mean no change).

The ratios were calculated by dividing RFU with aTc by RFU without aTc for each construct.

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