Regulatory

Part:BBa_J100302:Design

Designed by: Jackson Miller   Group: Campbell M Lab   (2016-09-08)


asr promoter (trimmed version of K1231000)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There was no recorded -10 or -35 sequence. We identified possible -10 sequences and used a position weight matrix to determine the most likely -10 sequence. Then we determined the -35 position by counting to the left 25 nucleotides. We cut the promoter to 56 bases that contained both consensus sequences. We inputed that sequence into the Oligator which added on the sticky ends according to the Golden Gate Assembly method.


Source

Registry of Standard Biological Parts: Part:BBa_K1231000

References