Measurement

Part:BBa_J100289

Designed by: Shuk Hang Li   Group: Campbell M Lab   (2016-07-12)


Pnar7 Nitrate Biosensor

This construct is built by modifying the promoter sequence of Original Nitrate Biosensor (J100237), which inserts narG promoter sequence into pClone Red (J119137) through GGA. The promoter sequence is modified by disrupting both Fis binding sites where an inhibitory regulator binds; moreover,since there is an overlap between the activator and inhibitor binding site, we had to make sure the activator can still bind on the same site. The modified promoter is inserted in to pClone Red (J119137). The goal is that nitrate induces the promoter, then RFP is expressed when nitrate is present. However, this promoter does not induce RFP production when nitrate is present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1677
    Illegal AgeI site found at 1789
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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