Plasmid

Part:BBa_J100287:Design

Designed by: Owen Koucky   Group: Campbell M Lab   (2016-07-10)


J100265 (pJC173b) with GFP replacing LuxAB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2481
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4050


Design Notes

We sought to replace LuxAB with GFP because we have greater familiarity with GFP fluorescence than we do with LuxAB luminescence.


Source

"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014 with modifications made by Hartlee Johnston in A. Malcolm Campbell's lab at Davidson College.

References