Device

Part:BBa_J100272:Experience

Designed by: Malcolm Campbell   Group: Campbell_M_Lab   (2016-06-21)


The problem with rClone Red v1 [J119384] was that the RBS we used was complementary to an "anti-RBS" located in RFP.

RCloneRedv1 antiRBS.png


To fix this problem, we mutated three codons so that they encoded the same 3 amino acids, but the resulting mRNA would not form the hairpin loop because we had disrupted the anti-RBS (Eckdahl et al., 2017).

RCloneRedv2 antiRBS.png


By removing the anti-RBS, the same RBS sequence now worked 10 times better than it had in rClone Red v1.

RCloneRedv2 data.png


We recommend everyone use J100272, rClone Red v2 as the preferred plasmid.


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