Coding

Part:BBa_J100238:Design

Designed by: Nicholas Elder   Group: Campbell M Lab   (2015-11-09)


tCloneTetRed with short stuffer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 290
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 436
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 462
    Illegal NgoMIV site found at 830
    Illegal NgoMIV site found at 990
    Illegal AgeI site found at 1937
    Illegal AgeI site found at 2049
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When tCloneTetRed is used to test riboswitches, there are approximately 80 bases added between the promoter and ribosome binding site. To test the efficiency of the system without the increased distance between the promoter and RBS, we synthesized this short stuffer sequence to insert into the part to test the 'natural' activity of the system.

Source

The part J119386 comes from J119361 tCloneRed and J119140 TetA. The linker sequence was developed from K598018 tetA+GFP fused protein by Qingyang XIAO. The short stuffer sequence is 5' GTGATCCTACA.

References