![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_J100223:Design
Designed by: Julia Preziosi Group: Campbell_M_Lab (2015-07-07)
Caffeine Aptamer with RS3, riboswitch in tCloneRed
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 821
Illegal AgeI site found at 933 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to add the aptamer to a riboswitch so that we could get the selective termination via the stem-loop behavior. We therefore did't know how the total riboswitch would fold or if it would be a successful riboswitch. Aptamer alone = GGAUGUCCAGUCGCUUGCAAUGCCCUUUUAGACCCUGAUGAGGAUCAUCGGACUUUGUCCUGUGGAGUAAGUCGCGAAACGGUGAAAGCCGUAGGUCU
Source
tCloneRed = BBa_J119361 Riboswitch 3 = J100207; Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ .