Device

Part:BBa_J100220:Design

Designed by: Julia Preziosi   Group: Campbell M Lab   (2015-06-24)


tCloneTet with Riboswitch 4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 356
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 502
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 528
    Illegal NgoMIV site found at 896
    Illegal NgoMIV site found at 1056
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The plasmid that this part is located in is pUC-BR, a plasmid modified from pUC-IDT-Amp with an errant BsaI site in the Ampicillin resistance gene removed.



Source

Part BBa_J119374, and Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ .

References