Device
Part:BBa_J100215:Design
Designed by: Julia Preziosi Group: Campbell M Lab (2015-06-24)
tCloneTet+Red fusion protein reporter with Riboswitch 4
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 356
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 502
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 528
Illegal NgoMIV site found at 896
Illegal NgoMIV site found at 1056
Illegal AgeI site found at 2003
Illegal AgeI site found at 2115 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The plasmid that this part is located in is pUC-BR, a plasmid modified from pUC-IDT-Amp with an errant BsaI site in the Ampicillin resistance gene removed.
Source
Part BBa_J119386, and Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ . This is the same riboswitch as is included in part J100207.