Composite
Part:BBa_J100205:Design
Designed by: Monica Prudencio Group: Campbell M Lab (2014-12-07)
repClone Red
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 9
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 9
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 9
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 9
Illegal AgeI site found at 2288
Illegal AgeI site found at 2400 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1638
Illegal BsaI.rc site found at 1517
Design Notes
The stop codons of the RBS and start codons of the proteins overlap to increase effective translation. This is cloned into pUC-IDT-Amp (BsaI site removed) with modified Bba prefix so that there is only an EcoRI site upstream and a normal Bba suffix.
Source
The two main indicator proteins are GFP (BBa_I746916) and RFP (BBa_E1010).