Regulatory
JEAN

Part:BBa_J100183:Design

Designed by: Erin Xu, Nick Elder, Julia Preziosi, Abby Hunt   Group: Campbell M Lab   (2014-09-11)

P R/tetO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed the sticky ends to be inducible with BsaI.


Source

The genomic sequence comes from rhizobial phage 16-3.

References

This sequence was adopted from "A novel pair of inducible expression vectors for use in Methylobacterium extorquens" by Lon M Chubiz, Jessica Purswani1, Sean Michael Carroll and Chistopher J Marx.

The paper can be found at the below URL: http://www.biomedcentral.com/1756-0500/6/183