Generator

Part:BBa_J100136:Design

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2014-02-20)


T5+riboswitchD+thyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 763
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used golden gate assembly to put the thyA gene into T5+riboswitch into pSB1K8 plasmid. We used BsaI restriction enzyme.


Source

composite device from existing parts

References