DNA

Part:BBa_J100114:Design

Designed by: Phoebe Parrish   Group: Campbell M Lab   (2013-07-13)


eCDM8 and GFP Riboswitch into pSB1A8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 237
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 196
    Illegal AgeI site found at 857
    Illegal AgeI site found at 3371
    Illegal AgeI site found at 3455
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3443


Design Notes

This construct was built using iPCR primers to amplify the promoter/RBS/eCDM8 construct (J119303) on pSB1A8 and add to BsaI sites to the end of the amplified sequence. This amplification removed the stuffer and the SpeI site from pSB1A8. PCR primers were used to amplify the promoter/riboswitch/GFP construct (J100079) and to add BsaI sites to the end of this sequence. GGA was then used to ligate these two amplified parts together.


Source

All parts taken from the registry.

References