Part:BBa_J100113:Design

sfGFP cloned into J100112

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 191
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

No internal BsaI sites. The 5' end of Junction D from part BBa_J119301 was changed from TTAA to CGGA. The change was made by iPCR with primers that caused the amplicon to contain the new sequence as shown in part J100113.

Source

All parts taken from Registry.