Coding
Part:BBa_J100107:Design
Designed by: Malcolm Campbell Group: Campbell M Lab (2013-03-12)
E. coli adhE gene
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 2679
Illegal PstI site found at 127
Illegal PstI site found at 462
Illegal PstI site found at 2028
Illegal PstI site found at 2617 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 2680
Illegal PstI site found at 127
Illegal PstI site found at 462
Illegal PstI site found at 2028
Illegal PstI site found at 2617
Illegal PstI site found at 2694
Illegal NotI site found at 2687 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 494
- 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 2680
Illegal PstI site found at 127
Illegal PstI site found at 462
Illegal PstI site found at 2028
Illegal PstI site found at 2617 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 2680
Illegal PstI site found at 127
Illegal PstI site found at 462
Illegal PstI site found at 2028
Illegal PstI site found at 2617
Illegal PstI site found at 2694 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1701
Design Notes
Removed four internal PstI sites and engineered this to be cloneable with GGA.
Source
Escherichia coli str. K-12 substr. MG1655 chromosome, complete genome NCBI Reference Sequence: NC_000913.2 >gi|49175990:1294669-1297344 Escherichia coli str. K-12 substr. MG1655 chromosome, complete genome The gene is encoded on the minus strand so we took reverse complement of the top strand.