Coding

Part:BBa_J100107:Design

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2013-03-12)


E. coli adhE gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2679
    Illegal PstI site found at 127
    Illegal PstI site found at 462
    Illegal PstI site found at 2028
    Illegal PstI site found at 2617
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2680
    Illegal PstI site found at 127
    Illegal PstI site found at 462
    Illegal PstI site found at 2028
    Illegal PstI site found at 2617
    Illegal PstI site found at 2694
    Illegal NotI site found at 2687
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 494
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2680
    Illegal PstI site found at 127
    Illegal PstI site found at 462
    Illegal PstI site found at 2028
    Illegal PstI site found at 2617
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2680
    Illegal PstI site found at 127
    Illegal PstI site found at 462
    Illegal PstI site found at 2028
    Illegal PstI site found at 2617
    Illegal PstI site found at 2694
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1701


Design Notes

Removed four internal PstI sites and engineered this to be cloneable with GGA.


Source

Escherichia coli str. K-12 substr. MG1655 chromosome, complete genome NCBI Reference Sequence: NC_000913.2 >gi|49175990:1294669-1297344 Escherichia coli str. K-12 substr. MG1655 chromosome, complete genome The gene is encoded on the minus strand so we took reverse complement of the top strand.


References