Generator

Part:BBa_J100106:Design

Designed by: Erin McGuire   Group: Campbell M Lab   (2013-02-19)


Riboswitch and GFP +Low production of eCDM8


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 4162
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 4163
    Illegal PstI site found at 4177
    Illegal NotI site found at 4170
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 4163
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 4163
    Illegal PstI site found at 4177
    Illegal AgeI site found at 100
    Illegal AgeI site found at 184
    Illegal AgeI site found at 1083
    Illegal AgeI site found at 1744
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 172


Design Notes

J100079 was ligated using Spe1 and J100105 using Xba1, so there is no scar between the two parts and EcoR1.


Source

eCDM8 was synthesized de novo.

References