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We mixed the E.coli sample with a growth medium then we pipetted the mixtures into individual wells. Once all the cells were pipetted we took the tray with seven samples and used a synergy machine to measure the RFP for each culture of cells. After we gathered the data from the machine and divided the fluorescence by the cell density to obtain the RFP fluorescence in each population. The table below displays the calculated RFP for the positive control (J04450) and the negative control (J100091) in addition to the other samples. Small represents small E.coli colonies and large represents large E.coli colonies.
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