Regulatory

Part:BBa_J100072

Designed by: Ben Clarkson   Group: Campbell M Lab   (2012-07-05)

LcpxP promoter--Long cpxP promoter

The promoter for the cpxP gene in E. coli.

Involved in responses to cytoplasmic stresses ([http://ecocyc.com/ECOLI/NEW-IMAGE?type=GENE-IN-MAP&object=G7816 cpxP gene in E. coli]). Transcription is activated by high extracellular pH via the CpxAR two-component signal transduction pathway.

Alkaline pH activates CpxA, an autokinase, which then phosphorylates CpxR. CpxR then activates cpxP and degP. DegP degrades cpxP, and cpxP inhibits the autokinase activity of CpxA ([http://ecocyc.com/ECOLI/NEW-IMAGE?type=ENZYME&object=PHOSPHO-CPXR CpxAR two-component signal transduction pathway in E. coli]).

Cloned into pSB1A8.

Similar to K339006.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None