Part:BBa_J100036:Experience

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Applications of BBa_J100036

This graph shows total florescence adjusted for cell density. Sample 1 was this polB promoter without any form of induction. Sample 2 was also this polB promoter, induced by adding sodium azide to cause DNA damage. Sample 3 was a negative control without a promoter. Instead it had a transcription terminator which prevented RFP from being produced. Sample 4 was a positive control in the form of constitutive promoter pTet. Sample 5 included the promoter pLacI with IPTG. Sample 6 was pLacI without IPTG. Our experimental promoter in the first tube was not induced and thus produced negligent amounts of red fluorescent protein. In contrast our induced experimental promoter produced a statistically significant amount of fluorescence.

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