RNA

Part:BBa_J04606:Design

Designed by: Nicholas Cain, Davidson College   Group: iGEM2005   (2005-06-28)


Antiswitch repressing Biobrick coding sequence at the RBS ("Off" switch)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 94
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 94
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 94
    Illegal BamHI site found at 55
    Illegal XhoI site found at 124
    Illegal XhoI site found at 227
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 94
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 94
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Transcription of the antiswitch is repressed in the presence of tetR. Also, the actual RBS sequence of BBa_B0034 is only 12 bp long; three additional base pairs comprise the total target sequence, and these additional 3 bp's correspond to the last three base pairs of the B0034 Biobrick Prefix, Xba I.


Source

Adaptation of antiswitch design "S1" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html

References