Reporter
Part:BBa_J04430:Design
Designed by: Kristen DeCelle Group: iGEM2005 (2005-06-09)
GFP coding device switched on by IPTG
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 870
Design Notes
Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.
Fwd: GCTTCTAGAGATACATGAAGATGCACTTcaatacgcaaaccgcctctccc
Rv: CTGCAGCGGCCGCTACTAGTAAGGAGCGCTTtataaacgcagaaaggcccacccg
Source
Davidson Synth-Aces