Part:BBa_I769901:Design

Thermoanaerobacter tengcongensis SASP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The gene sequence is the reverse complement of the appropriate segment of the whole genome sequence [complement(AE013046.1:4931..5236)].

Source

Thermoanaerobacter tengcongensis MB4. Sequence taken from GenBank:[gi:20807277].AE013046. Many other SASP sequences are available in GenBank, allowing for selection of GC-content, coding bias, etc. The choice of the T. tencongensis protein was based on its greater-than-average length and relatively recent availability from a whole-genome sequencing project.

References

Bao,Q., Tian,Y., Li,W., Xu,Z., Xuan,Z., Hu,S., Dong,W., Yang,J., Chen,Y., Xue,Y., Xu,Y., Lai,X., Huang,L., Dong,X., Ma,Y., Ling,L., Tan,H., Chen,R., Wang,J., Yu,J. and Yang,H. (2002) "A Complete Sequence of the T. tengcongensis Genome," Genome Res. 12 (5), 689-700.

Setlow, P. (2007) "I will survive: DNA protection in bacterial spores," Trends Microbiol. 15, 172-180.