Generator

Part:BBa_I766685:Design

Designed by: alexander ng   Group: iGEM07_UCSF   (2007-08-16)


YopJ-EE under inducible promotor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1370
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1370
    Illegal NotI site found at 2810
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2026
    Illegal BamHI site found at 1867
    Illegal BamHI site found at 2793
    Illegal XhoI site found at 1010
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1370
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1370
    Illegal NgoMIV site found at 172
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1795
    Illegal SapI.rc site found at 1819


Design Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonucleases AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.


Source

composite part

References