Part:BBa_I766212:Design

Ste2-GFP-HMTM1 under endogenous promoter

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3736
  Illegal EcoRI site found at 4434
  Illegal XbaI site found at 2546
  Illegal XbaI site found at 3389
  Illegal XbaI site found at 7407
  Illegal PstI site found at 2123
  Illegal PstI site found at 4785
  Illegal PstI site found at 7000
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3736
  Illegal EcoRI site found at 4434
  Illegal PstI site found at 2123
  Illegal PstI site found at 4785
  Illegal PstI site found at 7000
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3736
  Illegal EcoRI site found at 4434
  Illegal BglII site found at 3152
  Illegal BamHI site found at 4886
  Illegal BamHI site found at 8085
  Illegal XhoI site found at 2322
  Illegal XhoI site found at 3055
  Illegal XhoI site found at 3133
  Illegal XhoI site found at 3335
  Illegal XhoI site found at 4519
  Illegal XhoI site found at 7191
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3736
  Illegal EcoRI site found at 4434
  Illegal XbaI site found at 2546
  Illegal XbaI site found at 3389
  Illegal XbaI site found at 7407
  Illegal PstI site found at 2123
  Illegal PstI site found at 4785
  Illegal PstI site found at 7000
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3736
  Illegal EcoRI site found at 4434
  Illegal XbaI site found at 2546
  Illegal XbaI site found at 3389
  Illegal XbaI site found at 7407
  Illegal PstI site found at 2123
  Illegal PstI site found at 4785
  Illegal PstI site found at 7000
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1729
  Illegal BsaI site found at 6606
  Illegal BsaI.rc site found at 2976
  Illegal BsaI.rc site found at 7837
  

Design Notes

none

Source

composite