Composite

Part:BBa_I756031:Design

Designed by: Omar Khan   Group: iGEM07_Bay_Area_RSI   (2007-10-25)


mLexA-dnaEn-p85alpha


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 607
    Illegal XbaI site found at 3258
    Illegal PstI site found at 651
    Illegal PstI site found at 1555
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 607
    Illegal PstI site found at 651
    Illegal PstI site found at 1555
    Illegal NotI site found at 633
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 607
    Illegal BglII site found at 537
    Illegal BamHI site found at 617
    Illegal XhoI site found at 640
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 607
    Illegal XbaI site found at 3258
    Illegal PstI site found at 651
    Illegal PstI site found at 1555
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 607
    Illegal XbaI site found at 3258
    Illegal PstI site found at 651
    Illegal PstI site found at 1555
    Illegal NgoMIV site found at 1076
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2812


Design Notes

A flexible GS Linker (GGGGS)x5 (or more) is required between the dnaEc and Syk. This was quite difficult to clone in with the FseI site used in the part.


Source

p85 from OpenBiosystems (Clone ID: 3979333) mLexA from pEG202 dnaEn from dnaE gene of Synechocystis sp.(split inteins)

References