Reporter

Part:BBa_I756027:Design

Designed by: Jennifer Lei   Group: iGEM07_Bay_Area_RSI   (2007-10-25)


LexAopMLP-GFP-SV40PA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 988
    Illegal PstI site found at 994
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 994
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 961
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 988
    Illegal PstI site found at 994
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 988
    Illegal PstI site found at 994
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LexAop is a repeated sequence which results in multiple products. Isolate the largest fragment for gel isolation


Source

Mfe1, ClaI (1-12bp) LexAopMLP (minimal promoter) from pSH1834 plasmid (13-219bp) Asc1 (220-227bp) Kozak box (228-233bp) GFP from PWPI lentiviral plasmid (235-981bp) STOP STOP (983-987bp) Xba1 (988-993bp) Pst1 (994-999) SV40PA from pcDNA3.1 zeo (+) plasmid (1000-1130bp) HIndIII (1131-1136bp)

Digested with mfe1 and hind3 and cloned into puc18 digested with EcoR1 and HindIII.

References