Composite

Part:BBa_I751011

Designed by: Kenichiro Iwasaki   Group: iGEM07_Tokyo_Tech   (2007-10-23)

pcI-lux-lac-mCherry on pBR Standard

This is made for A-side plasmid in our project.

Expression is detected with the fluorescence protein mCherry and repressed by lambda cI.

This part is designed to move to the B side in our project by digesting with DraIII or AatII to adjaust the repression rate on the both sides.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2762
    Illegal suffix found in sequence at 5120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2762
    Illegal SpeI site found at 5121
    Illegal PstI site found at 5135
    Illegal NotI site found at 2768
    Illegal NotI site found at 5128
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2762
    Illegal BglII site found at 2890
    Illegal BglII site found at 3572
    Illegal BamHI site found at 2798
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2762
    Illegal suffix found in sequence at 5121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2762
    Illegal XbaI site found at 2777
    Illegal SpeI site found at 5121
    Illegal PstI site found at 5135
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2003
    Illegal SapI site found at 920


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