Part:BBa_I746210:Experience

It is not known whether this device actually causes cells to generate and export usable AIP or not, since an assay for AIP was never designed. They were tested with the reciever device built (I746220) but this produced a negative result. This was done in several ways. Firstly, overnight cultures of recievers were diluted 1:100, grown for 2 hours and then induced with varying amounts of supernatant from overnight sender cultures. They were viewed after two hours on a fluorescence microscope. A variation on this was to induce and then use a plate reader to take readings. Finally, a lawn of recievers was prepared by diluting 100ul of culture into 3ml of molten top agar, and induction was by spotting supernatant or sender cell culture onto a filter paper disc in the centre of the lawn. None of these tests showed induction.

It is not known which part of the system is at fault.

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