Part:BBa_I746201:Experience

The device was tested for its ability to confer sensitivity to the antibiotic bacitracin. Normally E. coli are resistant to this as it cannot pass through the outer membrane. With this device, the bacteria are killed by the antibiotic, evidenced by a ring of clearing seen when a bacitracin soaked disc is placed onto a lawn of growing bacteria. This suggests that the device confers non-selective permeability on molecules at least 1400Da in size.

Applications of BBa_I746201

The device was expressed in E. coli that were meant to be producing or detecting AIP. However, when these were tested, by seeing if the AIP senders could induce the detectors to produce more GFP, no change was seen. It is not known which part of the system was faulty in this test.

User Reviews

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