Signalling

Part:BBa_I746008:Design

Designed by: Lovelace Soirez   Group: iGEM07_Cambridge   (2007-10-23)


I0500 (Inducible pBad/araC) + I746001 (AIP generator - agrB + agrD with RBSes + Terminator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

N/A


Source

The original sequences for agrB and agrD were taken from the S. aureus Sequencing Project at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.

References

  1. Novick, Ross, Projan, Kornblum, Kreiswirth and Moghazeh (1993) - Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule. The EMBO Journal Vol. 12 no.10 pp.3967-3975
  2. Ji, Beavis and Novick (1995) - Cell density control of staphylococcal virulence mediated by an octapeptide pheromone. Proc. Natl. Acad. Sci. USA Vol. 92, pp. 12055-12059, December 1995, http://www.pnas.org/cgi/reprint/92/26/12055.pdf?ck=nck
  3. Peng, Novick, Kreiswirth, Kornblum and Schlievert (1988) - Cloning, Characterization, and Sequencing of an Accessory Gene Regulator (agr) in Staphylococcus aureus. J. Bacteriol., 179, 4365-4372
  4. Waters and Bassler (2005) - Quorum Sensing: Cell-to-Cell Communication in Bacteria. Annu. Rev. Cell Dev. Biol. 2005 21:319–46, http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.cellbio.21.012704.131001
  5. Ji, Beavis and Novick (1997) - Bacterial Interference Caused by Autoinducing Peptide Variants. Science 276 (5321), 2027, 27 June 1997
  6. Philip MDowell, Zina Affas, Caroline Reynolds, Matthew T. G. Holden, Stewart J. Wood, Sandra Saint, Alan Cockayne, Philip J. Hill, Christine E. R. Dodd, Barrie W. Bycroft, Weng C. Chan, Paul Williams (2001) - Structure, activity and evolution of the group I thiolactone peptide quorum-sensing system of Staphylococcus aureus. Molecular Microbiology (2001) 41(2), 503–512, http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2958.2001.02539.x
  7. Lyona and Novick - Peptide signaling in Staphylococcus aureus and other Gram-positive bacteria
  8. NARSA (A) - http://ww.narsa.net/control/member/search?repositoryId=105
  9. B.A. Roe, Y. R. Tian, H. Jia, S. Li, S. Lin, S. Kenton, H. Lai, JD White, A. Dorman, F. Z. Najar, S. Clifton, V. Worrell and J. Iandolo (A) - Staphylococcus aureus Genome Sequencing Project, http://www.genome.ou.edu/staph.html (direct link to sequence data: ftp://ftp.genome.ou.edu/pub/staph/staph-2k.fa)
  10. NCBI Entrez Genome (A) - accession number CP000253, http://www.ncbi.nlm.nih.gov/sites/entrez?db=genome&cmd=Retrieve&dopt=Overview&list_uids=19213 (direct link to sequence data: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=CP000253 (untick the two "hide" checkboxes))
  11. Koenig, Robbin L., Ray, Jessica L., Maleki, Soheila J., Smeltzer, Mark S., Hurlburt, Barry K. (2004) - Staphylococcus aureus AgrA Binding to the RNAIII-agr Regulatory Region. J. Bacteriol. 2004 186: 7549-7555, http://jb.asm.org/cgi/content/full/186/22/7549
  12. L Rao, R K Karls, and M J Betley (1995) - In vitro transcription of pathogenesis-related genes by purified RNA polymerase from Staphylococcus aureus. Bacteriol. 1995 May; 177(10): 2609–2614, http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=176928
  13. Morfeldt, E., K. Tegmark, and S. Arvidson (1996) - Transcriptional control of the agr-dependent virulence gene regulator, RNAIII, in Staphylococcus aureus. Mol. Microbiol. 21:1227–1237