Coding

Part:BBa_I745004:Design

Designed by: MGC collection   Group: iGEM07_Princeton   (2007-10-26)


Bax gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 226
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 349
    Illegal PstI site found at 226
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 452
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 226
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 226
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Bax is PCR'd out of the original plasmid and SfiI sites added to either end.


Source

ATCC; mouse gene

References