Translational_Unit
rbs+sam8

Part:BBa_I742148:Design

Designed by: sarah hollingshead   Group: iGEM07_Edinburgh   (2007-10-24)


sam8 (tyrosine ammonia lyase) with native rbs


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 808
    Illegal BamHI site found at 1210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 551
    Illegal AgeI site found at 498
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 102
    Illegal BsaI site found at 368


Design Notes

This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt ligation in generating the final sequence.


Source

Saccharothrix espanaensis DSM 44229

References