Coding
sam5

Part:BBa_I742141:Design

Designed by: sarah hollingshead   Group: iGEM07_Edinburgh   (2007-10-23)


sam5 (coumarate hydroxylase) coding sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1420
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1420
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1420
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1420
    Illegal NgoMIV site found at 21
    Illegal NgoMIV site found at 29
    Illegal NgoMIV site found at 779
    Illegal NgoMIV site found at 952
    Illegal NgoMIV site found at 1354
    Illegal NgoMIV site found at 1358
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 553


Design Notes

Note that this version of the gene contains a PstI site near the 5' end. This will ultimately be removed by site directed mutagenesis.


Source

Saccharothrix espanaensis DSM 44229, sequence accession ABC88666.

References