Part:BBa_I742123:Design

Multi-host vector pTG262 converted to BioBrick vector

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5543
  Illegal NheI site found at 694
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 5549
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5543
  Illegal BglII site found at 3754
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 5543
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 5543
  Plasmid lacks a suffix.
  Illegal XbaI site found at 5558
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal AgeI site found at 3365
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI.rc site found at 3578
  

Design Notes

See [http://openwetware.org/wiki/Cfrench:BioBrickVectors1 notes on pTG262], the vector from which BBa_I742123 was derived.

Source

We kindly thank Dr Mike Gasson & Dr. Claire Shearman, of the Institute of Food Research in Norwich for generously donating the pTG262 vector.