Coding

Part:BBa_I738002:Design

Designed by: Kari Clase   Group: iGEM07_Purdue   (2007-05-26)


feruloyl-CoA hydratase enzyme for biosynthesis of vanillin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 811
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 811
    Illegal NotI site found at 755
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 811
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 811
    Illegal NgoMIV site found at 184
    Illegal NgoMIV site found at 202
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Changed stop codon from TGA to TAATAA BioBricks std


Source

GenBank Accession #: AJ536325 Reference: Berghini 2007 "Vanillin production using metabolically engineered Escherichia coli under non-growing conditions"

References