Plasmid

Part:BBa_I732958

Designed by: Zhan Jian   Group: iGEM07_USTC   (2007-10-26)

Ptemplate1 + RBS.ARL4A0604.TERM

Genotype Character Name
Top10/pT-TERM So not express any repressors CR00
Top10/pT-ARL4A0604 Constitutively express ARL4A0604 CR01
Top10/pT-ARL2A0203 Constitutively express ARL2A0203 CR10
Top10/pTet-ARL4A0604-ARL2A203 Constitutively express ARL4A0604 and ARL2A0203 CR11

USTC plasmid 958.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2209
    Illegal EcoRI site found at 4344
    Illegal XbaI site found at 4509
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4344
    Illegal SpeI site found at 2210
    Illegal PstI site found at 2224
    Illegal NotI site found at 2217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4344
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2210
    Illegal EcoRI site found at 4344
    Illegal XbaI site found at 4509
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4344
    Illegal XbaI site found at 4509
    Illegal SpeI site found at 2210
    Illegal PstI site found at 2224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3383
    Illegal BsaI.rc site found at 4368


[edit]
Categories
//plasmid
Parameters
chassis
copies
mcsBioBrick friendly
origin
resistanceAK