Regulatory
Pnot(O21)

Part:BBa_I732273

Designed by: Zhan Jian   Group: iGEM07_USTC   (2007-10-14)

Promoter Family Member with Hybrid Operator (D001O21)

Promoter family member with hybrid operator O21 placed at +10.5.

Usage

  • Measure [http://2007.igem.org?title=USTC/OperatorComposition the effect of operator composition]
  • [http://2007.igem.org?title=USTC/HybridOperator A hybrid operator]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 18


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter/logic/ustc
//regulation/negative
Parameters
negative_regulators1
positive_regulators