Coding

Part:BBa_I729002:Design

Designed by: Hiroki Fukutomi   Group: iGEM07_Chiba   (2007-10-21)


Flagellin　(Flic histag)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 802
    Illegal SpeI site found at 547
    Illegal PstI site found at 914
    Illegal PstI site found at 961
    Illegal PstI site found at 1528
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 802
    Illegal SpeI site found at 547
    Illegal PstI site found at 914
    Illegal PstI site found at 961
    Illegal PstI site found at 1528
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 802
    Illegal BamHI site found at 1266
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 802
    Illegal SpeI site found at 547
    Illegal PstI site found at 914
    Illegal PstI site found at 961
    Illegal PstI site found at 1528
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 802
    Illegal SpeI site found at 547
    Illegal PstI site found at 914
    Illegal PstI site found at 961
    Illegal PstI site found at 1528
    Illegal AgeI site found at 301
    Illegal AgeI site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Histag at 267 aa


Source

genomic PCR -> linker ligation


References