Part:BBa_I72512:Design

Enoyl-coa hydratase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 163
  Illegal AgeI site found at 294
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence has had its codons optimized for E. coli and has had its restriction sites removed. The sequence has had its stop codon changed to TAATAA. It has had a His tag added to its c terminus.

Source

C. acetobutylicum genome. CAC_2712