Composite

Part:BBa_I724005:Design

Designed by: Susan Westfall   Group: iGEM07_McGill   (2007-10-26)


Elowitz repressilator with added degradation tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2222
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Usage of BsrGI which cleaved the reporter in both the I5611 and E0434 thus putting the two pieces together.


Source

Due to the anolmalous gel results for the I5610 digestion, a new construct of the I5610 was made using I5611 (elowitz repressilator minus the LVA) and the LVA from E0434

References