Composite
Part:BBa_I724005:Design
Designed by: Susan Westfall Group: iGEM07_McGill (2007-10-26)
Elowitz repressilator with added degradation tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2222
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Usage of BsrGI which cleaved the reporter in both the I5611 and E0434 thus putting the two pieces together.
Source
Due to the anolmalous gel results for the I5610 digestion, a new construct of the I5610 was made using I5611 (elowitz repressilator minus the LVA) and the LVA from E0434