Intermediate

Part:BBa_I723031:Design

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-10-26)


Pu plus RBS plus Renilla luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 893


Design Notes

Source

Cloned from the TOL plasmid from Pseudomonas putida MT2. This is the promoter that regulates the TOL catabolic plasmid upper pathway operon.

RBS and luciferase from iGEM source plates.

References