Coding
XylR

Part:BBa_I723017:Design

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-10-25)


XylR coding region


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 643
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 466
    Illegal PstI site found at 643
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 86
    Illegal BglII site found at 1548
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 643
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 643
    Illegal NgoMIV site found at 252
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 739


Design Notes

It does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of XylR.

Source

Cloned from the TOL plasmid from Pseudomonas putida MT2

References

Worsey, M., and Williams, P. 1975. Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13.