Coding
XylR
Part:BBa_I723017:Design
Designed by: Scott Ramsay Group: iGEM07_Glasgow (2007-10-25)
XylR coding region
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 643
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 466
Illegal PstI site found at 643 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 86
Illegal BglII site found at 1548 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 643
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 643
Illegal NgoMIV site found at 252 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 739
Design Notes
It does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of XylR.
Source
Cloned from the TOL plasmid from Pseudomonas putida MT2
References
Worsey, M., and Williams, P. 1975. Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13.