Regulatory

Part:BBa_I720007:Design

Designed by: Andrew Cheng   Group: iGEM07_Michigan   (2007-10-26)


araBp->rpoN (leucine landing pad)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

rpoN has a PstI site. Therefore, to insert into the leucine landing pad, we cut with EcoRI and SpeI


Source

Both araBp and rpoN is from genomic E. Coli DNA.

References