Regulatory

Part:BBa_I720002:Design

Designed by: Andrew Cheng   Group: iGEM07_Michigan   (2007-10-26)


glnKp->lacI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 172
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 172
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 172
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 172
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No special considerations


Source

This part was previously built and inserted into E. Coli chromosome using the glnK landing pad. The part was PCR amplified from the genome and inserted into pSB1A2.

References