Temporary

Part:BBa_I720000:Design

Designed by: Andrew Cheng   Group: iGEM07_Michigan   (2007-10-26)


rpoN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal PstI site found at 989
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 989
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 601
    Illegal BamHI site found at 564
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal PstI site found at 989
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal PstI site found at 989
    Illegal AgeI site found at 473
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 260


Design Notes

rpoN has a PstI site, so construction of this part (and any future addtions) cannot use PstI as a restriction enzyme.


Source

...consult aaron's notes

References