Part:BBa_I718003:Design

FtsA-FtsZ

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 247
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 687
  Illegal AgeI site found at 1026
  Illegal AgeI site found at 1116
  Illegal AgeI site found at 1668
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

EcoR1 site in FtsZ deleted (gaattc -> gagttc)

Source

Escherichia coli K12 (strain: K-12, substrain: MG1655), genomic sequence